Membranes (Millipore, Shanghai, China) were incubated with antibodies against p38 MAPK, p-p38 MAPK, FOXO3a and IGFBP1 (1:1000). FOXO3a were completed by transient transfection assays. IGFBP1 promoter activity was assessed by Secrete-Pair? Dual Luminescence Assay Package. In vivo nude mice xenograft model and bioluminescent imaging program were used to verify the results in vitro. Outcomes We … Continue reading Membranes (Millipore, Shanghai, China) were incubated with antibodies against p38 MAPK, p-p38 MAPK, FOXO3a and IGFBP1 (1:1000)